Regulatory

Part:BBa_K4687048

Designed by: Yiming Jiang   Group: iGEM23_HBUT-China   (2023-10-12)


Promoter1

The designed promoter sequence was ligated with donor DNA to construct an expression vector. The expression vector was transformed into E. coli to amplify the plasmid and verified to be correct by sequencing, and then transformed into Corynebacterium glutamicum as a means of observing the expression effect of the system. It was observed whether the promoter could enhance the expression of system in Corynebacterium glutamicum.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 30
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal SpeI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 30
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 30
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None